Journal: Bioactive Materials

Article Title: Natural killer cell-inspired dendritic mesoporous rare-earth nanoparticles potentiate X-ray-triggered reactive oxygen generation for low-dose radiotherapy-radiodynamic therapy

doi: 10.1016/j.bioactmat.2026.02.011

Figure Lengend Snippet: In vitro targeted cell uptake and oxygen supply ability. (a) SDS-PAGE electrophoresis profiles and Western blot results of TSSI-Ce 6 C-DMTm, NKEV, and TSSI-Ce 6 C-DMTm@NKEV. (b) Scheme of the tumor-targeting mechanism of TSSI-Ce 6 C-DMTm@NKEV. (c) Western blot results of NK cells, NKEV, TSSI-Ce 6 C-DMTm@NKEV, and TSSI-Ce 6 C-DMTm to indicate the presence of DNAM-1 and NKG2D. (d) CLSM images and quantification analysis of MDA-MB-231 cells treated with TSSI-Ce 6 C-DMTm and TSSI-Ce 6 C-DMTm@NKEV for 1, 3, and 9 h. (f) Flow cytometry profiles and (g) CLSM images of MDA-MB-231 cells pretreated with different antibodies and treated with TSSI-Ce 6 C-DMTm@NKEV for 9 h. (h) CLSM images of MDA-MB-231 cells pretreated with H 2 O 2 and treated with different formulations via [Ru(dpp) 3 ]Cl 2 staining for hypoxia levels observation. ∗∗∗∗ p < 0.0001.

Article Snippet: Then, the membrane was blocked using 5% skim milk and incubated using primary antibody of anti -DNAM-1 (ABclonal, A23200), anti -NKG2D (Bioss, bs-0938R), anti -β-actin (Beijing Solarbio Science & Technology Co., Ltd.), anti-Na/K ATPase (Abcam, ab254025), respectively.

Techniques: In Vitro, SDS Page, Electrophoresis, Western Blot, Flow Cytometry, Staining

Journal: Materials Today Bio

Article Title: Ultrasound-responsive CPS piezoelectric hydrogel synergistically repairs annulus fibrosus defects through immune reprogramming and cell recruitment

doi: 10.1016/j.mtbio.2026.102825

Figure Lengend Snippet: Assessment of the anti-inflammatory capacity of CPS gel in vitro . A Schematic of the co-culture of hydrogel with RAW 264.7. B Quantification of the relative fluorescence intensity of TNF-α. C Quantification of the relative fluorescence intensity of IL-1β. D Immunofluorescence image of TNF-α expression in RAW 264.7 (Scale bar = 50 μm). E Immunofluorescence image of IL-1β expression in RAW 264.7 (Scale bar = 50 μm). F-H The protein expression level of TNF-α and IL-1β was evaluated by WB and quantified by ImageJ. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Staining was performed using IL-1β (Bioss, BS-0812R) and TNF-α (Santa, SC-52746) probes, and the staining was observed under a fluorescent inverted microscope.

Techniques: In Vitro, Co-Culture Assay, Fluorescence, Immunofluorescence, Expressing

Journal: Materials Today Bio

Article Title: Ultrasound-responsive CPS piezoelectric hydrogel synergistically repairs annulus fibrosus defects through immune reprogramming and cell recruitment

doi: 10.1016/j.mtbio.2026.102825

Figure Lengend Snippet: In vivo study of CPS gel for AF repair. A H&E, S&O staining sections of rat IVD at 4W and 8W post-operation (scale bar = 1 mm). B Col-1, IL-1β IHC staining sections of rat IVD at 4W and 8W post-operation (Scale bar = 50 μm). C Postoperative 4W Col-1 quantitative analysis. D Postoperative 8W Col-1 quantitative analysis. E. Postoperative 4W IL-1β quantitative analysis. F Postoperative 8W IL-1β quantitative analysis. Data are mean ± SD (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Staining was performed using IL-1β (Bioss, BS-0812R) and TNF-α (Santa, SC-52746) probes, and the staining was observed under a fluorescent inverted microscope.

Techniques: In Vivo, Staining, Immunohistochemistry

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Abnormal expression of antioxidant and iron metabolism-related proteins in liver samples of rats with chronic cholestasis. Control group was fed a chow diet, whereas the ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) Changes in ferroptosis antioxidant-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (B) Nrf2, (C) Keap1, (D) XCT, (E) HO-1 and (F) GPX4. (G) Changes in iron metabolism-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (H) TFR1, (I) FPN1, (J) DMT1, (K) Steap3 and (L) FTH1. Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001. ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine; Nrf2, nuclear factor erythroid-2-related factor 2; Keap1, Kelch-like ECH-associated protein 1, XCT, cystine/glutamate transporter; HO-1, heme oxygenase 1; GPX4, glutathione peroxidase 4; TFR1, transferrin receptor 1; FPN1, ferroportin 1; DMT1, divalent metal transporter 1; Steap3, six-transmembrane epithelial antigen of the prostate 3; FTH1, ferritin heavy chain 1.

Article Snippet: The Kelch-like ECH-associated protein 1 (Keap1) antibody (cat. no. bs-3648R) was purchased from BIOSS.

Techniques: Expressing, Control, Derivative Assay, Membrane

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Diagram of the mechanism of ferroptosis. ASCL4, acyl-CoA synthetase long-chain family member 4; COX2, cyclooxygenase 2; DMT1, divalent metal transporter 1; FPN, ferroportin; GSSG, oxidized glutathione; GPX4, glutathione peroxidase 4; GSH, glutathione; HO-1, heme oxygenase 1; Keap1, Kelch-like ECH-associated protein 1; LIP, labile iron pool; LPCAT3, lysophosphatidylcholine acyltransferase 3; NCOA4, nuclear receptor coactivator 4; NOX1, nicotinamide adenine dinucleotide phosphate oxidase 1; NQO1, NAD(P)H quinone dehydrogenase 1; Nrf2, nuclear factor erythroid-2-related factor 2; PL-PUFA-OOH, phospholipid-polyunsaturated fatty acid hydroperoxide; SLC7A11, solute carrier family 7 member 11; Steap3, six-transmembrane epithelial antigen of the prostate 3; TFR1, transferrin receptor 1.

Article Snippet: The Kelch-like ECH-associated protein 1 (Keap1) antibody (cat. no. bs-3648R) was purchased from BIOSS.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: ARF6 controls VSMC phenotypic switching upon lipid stimulation to promote inflammatory signaling contributing to the progression of atherosclerosis

doi: 10.1016/j.jbc.2026.111165

Figure Lengend Snippet: ARF6 knockout affects local inflammation in the plaque . A , images of aortic arch cross-sections stained with H&E. Arrows identify the foam cells. n = 5 animals per group. The scale bar represents 100 μm. B , total RNA was extracted from isolated mouse abdominal aortic VSMCs with the Qiagen RNeasy kit according to the manufacturer’s instructions. Quantifications are the mean ± SD of six or seven animals per group (three animals per group for Il1β ). ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05, ns, not significant (unpaired Student’s t test). C , representative aortic sinus cross-sections stained for immunohistochemistry with anti-CD45. n = 5 animals per group. The scale bar represents 250 μm. D , immunofluorescence of frozen aortic sinus stained with anti-CD68 and Alexa Fluor 568. Nuclei were stained with DAPI. Images are representative of three nonconstitutive sections per animal, n = 5 per group. Quantifications are the mean ± SD of six or eight lesions per group. ns, not significant (unpaired Student’s t test). E–F , immunofluorescence of frozen aortic sinus stained with anti-ICAM-1 ( E ) or anti-VCAM-1 ( F ) and Alexa Fluor 568. Nuclei were stained with DAPI. Images are representative of three nonconstitutive sections per animal, n = 5 per group. Quantifications are the mean ± SD of 8 to 11 lesions per group. ∗ p < 0.05 (unpaired Student’s t test). Dotted lines encircle the lesions. The scale bar represents 100 μm. ARF, ADP-ribosylation factorl; ICAM, intracellular adhesion molecule; VCAM, vascular cell adhesion molecule; VSMC, vascular smooth muscle cell.

Article Snippet: Anti-VCAM-1 (bs-0920R) was from Beijing Biosynthesis Biotechnology Co., Ltd (BIOSS).

Techniques: Knock-Out, Staining, Isolation, Immunohistochemistry, Immunofluorescence